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来自瘙痒病感染仓鼠脑的膜泡中核酸的特性分析

Characterization of nucleic acids in membrane vesicles from scrapie-infected hamster brain.

作者信息

Dees C, McMillan B C, Wade W F, German T L, Marsh R F

出版信息

J Virol. 1985 Jul;55(1):126-32. doi: 10.1128/JVI.55.1.126-132.1985.

DOI:10.1128/JVI.55.1.126-132.1985
PMID:2409296
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC254906/
Abstract

This study reports the partial characterization of nucleic acids present in gradient fractions enriched for large membrane vesicles from scrapie-infected and uninfected hamster brains. Labeling of phenol-extracted nucleic acids at the 3' or 5' ends revealed abundant amounts of low-molecular-weight RNA and little or no DNA. These nucleic acids survived nuclease treatment of membrane vesicles but were sensitive to RNase after phenol extraction. Analysis of 5'-end-labeled nucleic acids by one- and two-dimensional gel electrophoresis revealed an RNA of ca. 100 bases in preparations from scrapie-infected hamster brain that could not be detected in uninfected brain. The possibility that this apparently unique small RNA may result from tissue damage or abnormal RNA processing or may be a component of the infectious complex is discussed.

摘要

本研究报告了从感染羊瘙痒病和未感染羊瘙痒病的仓鼠脑中富集的大膜泡梯度级分中存在的核酸的部分特性。对酚抽提核酸的3'或5'末端进行标记，结果显示存在大量低分子量RNA，几乎没有或根本没有DNA。这些核酸在膜泡经核酸酶处理后仍能存活，但酚抽提后对核糖核酸酶敏感。通过一维和二维凝胶电泳对5'末端标记核酸进行分析，发现在感染羊瘙痒病的仓鼠脑制备物中有一条约100个碱基的RNA，而在未感染的脑中未检测到。本文讨论了这种明显独特的小RNA可能是由组织损伤或异常RNA加工导致的，或者可能是感染性复合物的一个组成部分的可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c3d/254906/e9106aee63d8/jvirol00118-0142-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c3d/254906/3e4a08a8d9ca/jvirol00118-0137-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c3d/254906/de7c80ada62e/jvirol00118-0137-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c3d/254906/b2a5de7a2267/jvirol00118-0139-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c3d/254906/e75227a4b6ea/jvirol00118-0139-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c3d/254906/e8d73f962243/jvirol00118-0140-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c3d/254906/a8b68f704018/jvirol00118-0140-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c3d/254906/e9106aee63d8/jvirol00118-0142-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c3d/254906/3e4a08a8d9ca/jvirol00118-0137-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c3d/254906/de7c80ada62e/jvirol00118-0137-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c3d/254906/b2a5de7a2267/jvirol00118-0139-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c3d/254906/e75227a4b6ea/jvirol00118-0139-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c3d/254906/e8d73f962243/jvirol00118-0140-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c3d/254906/a8b68f704018/jvirol00118-0140-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c3d/254906/e9106aee63d8/jvirol00118-0142-a.jpg

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引用本文的文献

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Evidence of mitochondrial involvement in scrapie infection.线粒体参与羊瘙痒病感染的证据。
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A 54-kDa normal cellular protein may be the precursor of the scrapie agent protease-resistant protein.一种54千道尔顿的正常细胞蛋白可能是羊瘙痒病病原体蛋白酶抗性蛋白的前体。

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