Chen Qian, Sun Yong, Wang Jiangjun, Yan Guowen, Cui Zhaoyuan, Yin Hongli, Wei Haitian
School of Pharmacy, Qingdao University , Qingdao , P. R. China.
Artif Cells Nanomed Biotechnol. 2015;43(4):217-23. doi: 10.3109/21691401.2013.845570. Epub 2013 Oct 4.
Stearic acid-grafted chitosan (CS-SA) and glycyrrhetinic acid-conjugated stearic acid-grafted chitosan (GA-CS-SA) were synthesized and were further used for the preparation of micelles. The substitution degree (SD) of SA and GA on CS was measured. The physicochemical properties of CS-SA and GA-CS-SA micelles such as critical micelle concentration (CMC), aggregation number of hydrophobic micro-domain (AN), particle size, zeta potential, and morphology were also determined. The CMC of GA-CS-SA was about 17.49 μg/mL, which was relatively low. Its AN was 2.09. The GA-CS-SA micelles showed spherical shape with mean diameter of 121.1 nm and had positive charge, which suggested that GA-CS-SA could be a good carrier of cancer drug.
合成了硬脂酸接枝壳聚糖（CS-SA）和甘草次酸共轭硬脂酸接枝壳聚糖（GA-CS-SA），并将其进一步用于制备胶束。测定了SA和GA在CS上的取代度（SD）。还测定了CS-SA和GA-CS-SA胶束的物理化学性质，如临界胶束浓度（CMC）、疏水微区聚集数（AN）、粒径、zeta电位和形态。GA-CS-SA的CMC约为17.49μg/mL，相对较低。其AN为2.09。GA-CS-SA胶束呈球形，平均直径为121.1nm，带正电荷，这表明GA-CS-SA可能是一种良好的抗癌药物载体。
