School of Ecological Technology and Enginneering, Shanghai Institute of Technology, Shanghai 201418, China.
Plant Sci. 2013 Nov;212:48-54. doi: 10.1016/j.plantsci.2013.05.017. Epub 2013 Jun 8.
A PCR strategy was used to isolate a full-length CgADH (alcohol dehydrogenase) cDNA from chrysanthemum. The gene putatively encodes a 378 residue polypeptides, which shares 95% homology with tomato alcohol dehydrogenase class III. Endogenous ethylene generated in waterlogged Chrysanthemum zawadskii was enhanced by exogenous ethylene but decreased by 1-methylcyclopropene (1-MCP), an inhibitor of ethylene action. In waterlogged roots, the transcription of the gene encoding alcohol dehydrogenase (ADH, EC 1.1.1.1) increased rapidly but transiently, peaking at 7.5 fold the non-waterlogged level after 2h of stress. Waterlogging elevated ADH activity after a prolonged episode of stress. The exogenous supply of 40μLL(-1) ethylene suppressed the production of ethanol, while that of 4μLL(-1) 1-MCP enhanced it. Ethylene appeared to suppress an acceleration of both CgADH expression and fermentation, and alleviates ethanolic fermentation probably through by as a signal to acceleration of waterlogging-induced aerenchyma formation. This supports the previously observed phenomenon that the expression level of ADH gene is regulated by the local level of physiologically active ethylene. The relevance of the CgADH gene in relation to chrysanthemum waterlogging was discussed as well.
采用 PCR 策略从菊花中分离出全长 CgADH（醇脱氢酶）cDNA。该基因推测编码一个 378 个残基的多肽，与番茄醇脱氢酶 III 类具有 95%的同源性。水淹胁迫下，内源乙烯在菊花中的生成增强，但外源乙烯处理下降低，1-甲基环丙烯（1-MCP）作为乙烯作用的抑制剂，也会降低内源乙烯的生成。在水淹根中，编码醇脱氢酶（ADH，EC 1.1.1.1）的基因转录迅速但短暂增加，在胁迫 2 小时后达到非水淹水平的 7.5 倍。长时间水淹胁迫后，ADH 活性升高。外源 40μLL(-1)乙烯的供给抑制了乙醇的生成，而 4μLL(-1) 1-MCP 的供给则增强了乙醇的生成。乙烯似乎抑制了 CgADH 表达和发酵的加速，并通过作为加速水淹诱导通气组织形成的信号来缓解乙醇发酵。这支持了先前观察到的 ADH 基因的表达水平受局部生理活性乙烯水平调节的现象。还讨论了 CgADH 基因与菊花水淹的关系。
