Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research), 3 Research Link, Singapore 117602, Singapore.
Mater Sci Eng C Mater Biol Appl. 2013 Dec 1;33(8):4545-50. doi: 10.1016/j.msec.2013.07.011. Epub 2013 Jul 17.
The synthesis and gene transfection efficiency of a series of amphiphilic copolymers with poly(dimethylaminoethyl methacrylate) (PDMAEMA), and poly(propylene glycol methacrylate) (PPGMA) segments is reported. The hydrophobic PPGMA interior allows a cell-sensitizing drug such as paclitaxel to be incorporated while the cationic and hydrophilic PDMAEMA corona allows the complexation of anionic DNA to form a nano-sized polyplex. These drug-encapsulated copolymers display excellent gene transfection efficiency as compared to PEI or PDMAEMA homopolymers.
本文报道了一系列具有聚（二甲基氨基乙基甲基丙烯酸酯）（PDMAEMA）和聚（丙二醇甲基丙烯酸酯）（PPGMA）段的两亲性共聚物的合成及其基因转染效率。疏水性 PPGMA 内核允许包裹如紫杉醇等细胞敏感药物，而带正电荷和亲水性 PDMAEMA 冠层则允许与阴离子 DNA 复合形成纳米级的聚阳离子。与 PEI 或 PDMAEMA 均聚物相比，这些包载药物的共聚物显示出优异的基因转染效率。
