Department of Organic Materials and Fiber Engineering, Chonbuk National University, Jeonju 561-756, South Korea.
Mater Sci Eng C Mater Biol Appl. 2013 Dec 1;33(8):5051-7. doi: 10.1016/j.msec.2013.08.032. Epub 2013 Sep 4.
The biocompatible and highly porous keratin-based hydrogels were prepared using electron beam irradiation (EBI). The conditions for keratin-based hydrogel formation were investigated depending on several conditions, including the presence of poly(vinyl alcohol) (PVA), concentration of keratin solution, EBI dose, and poly(ethylene imine) (PEI) additives. The pure keratin (human hair and wool) aqueous solution was not gelled by EBI, while the aqueous keratin solutions blended with PVA were gelled at an EBI dose of more than 90 kGy. Furthermore, in the presence of PEI, the aqueous keratin solution blended with PVA could be gelled at a considerably lower EBI dose, even at 10 kGy. This finding suggests that the PEI additives significantly influence the rate of gelation and that PEIs function as an accelerator during gelation. The resulting keratin-based hydrogels were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), gel fraction, degree of swelling, gel strength, and kinetics of swelling analyses.
采用电子束辐照（EBI）制备了具有生物相容性和高多孔性的角蛋白基水凝胶。根据多种条件研究了角蛋白水凝胶形成的条件，包括存在聚乙烯醇（PVA）、角蛋白溶液的浓度、EBI 剂量和聚乙烯亚胺（PEI）添加剂。纯角蛋白（人发和羊毛）水溶液不能通过 EBI 凝胶化，而与 PVA 混合的角蛋白水溶液在 EBI 剂量超过 90 kGy 时可以凝胶化。此外，在存在 PEI 的情况下，与 PVA 混合的角蛋白水溶液即使在 10 kGy 的低 EBI 剂量下也可以凝胶化。这一发现表明，PEI 添加剂显著影响凝胶化速率，并且 PEI 在凝胶化过程中起促进剂的作用。使用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）、凝胶分数、溶胀度、凝胶强度和溶胀动力学分析对所得角蛋白基水凝胶进行了表征。
