Kumar Kuldeep, Singh Renu, Kumar Manish, Agarwal Pranjali, Mahapatra P S, Kumar Ajay, Malakar Dhruba, Bag Sadhan
1Reproductive Physiology Lab, Division of Physiology & Climatology, Indian veterinary Research Institute , Izatnagar, Bareilly, Uttar Pradesh , India.
Int J Neurosci. 2014 Jun;124(6):450-6. doi: 10.3109/00207454.2013.852087. Epub 2013 Nov 7.
Neural stem cells (NSCs) are primordial, uncommitted cells postulated to give rise to the array of more specialized cells of the central nervous system (CNS). NSCs can self-renew and give rise to neurons, astrocytes and oligodendrocytes. NSCs are found in the CNS of mammalian organisms, and represent a promising resource for both fundamental research and CNS repair. Animal models of CNS damage have highlighted the potential benefit of NSC-based approaches. Present study described that buffalo neural stem cells (Bu-NSCs) were isolated and expanded rapidly from buffalo fetal brain in adherent culture. They were capable of multidifferentiation into neurons, astrocytes, and oligodendrocytes. Bu-NSCs were morphologically homogeneous and possessed high proliferation ability. The population doubled every 128.16 h. Normal buffalo karyotype was unchanged throughout the in vitro culture period. Together, we have isolated and cultured Bu-NSC from fetal brain that showed self-renewal, rapid proliferation and ability to differentiate into cells of nervous system. The availability of such cells may hold great interest for basic and applied neuroscience.
神经干细胞（NSCs）是原始的、未分化的细胞，被认为可产生中枢神经系统（CNS）中一系列更特化的细胞。神经干细胞能够自我更新，并产生神经元、星形胶质细胞和少突胶质细胞。神经干细胞存在于哺乳动物机体的中枢神经系统中，是基础研究和中枢神经系统修复的一个有前景的资源。中枢神经系统损伤的动物模型突出了基于神经干细胞方法的潜在益处。目前的研究描述了水牛神经干细胞（Bu-NSCs）在贴壁培养中从水牛胎儿脑中被快速分离和扩增。它们能够多向分化为神经元、星形胶质细胞和少突胶质细胞。水牛神经干细胞在形态上是均匀一致的，并且具有高增殖能力。群体倍增时间为每128.16小时。在整个体外培养期间，正常水牛核型未发生改变。总之，我们从胎儿脑中分离并培养了水牛神经干细胞，其表现出自我更新、快速增殖以及分化为神经系统细胞的能力。这类细胞的可用性可能会引起基础神经科学和应用神经科学的极大兴趣。
