Karabük Govermental Hospital, Department of Pediatric Surgery, Karabük, Turkey.
J Pediatr Surg. 2013 Oct;48(10):2164-70. doi: 10.1016/j.jpedsurg.2013.06.030.
This study aimed to evaluate the effect of P/E-selectin blockage on antisperm antibody (ASA) development and histopathological alterations in experimental orchitis.
Thirty-six Wistar albino-type male rats weighing 100-150 g were included in the study. Rats were allocated into six groups (n = 6) including control (CG), sham (SG), orchitis (OG), antimicrobial treatment (AG), P/E-selectin blockage (PESG), and both antimicrobial and P/E-selectin treatment (TG) groups. In CG, serum samples were taken from the tail vein prior to the procedure and followed by extraction of both testes. In SG, 1 ml of saline solution was injected in testicular parenchyma. OG was obtained by injecting 0.1 ml 106 cfu/ml Escherichia coli (0:6 strain) and 1 ml saline solution into the right testes. AG received ciprofloxacin (50 mg/kg/day) twice a day through gastrogavage 24 hours after generating orchitis. In PESG, P/E-selectin antibody (100 μg) was administered intravenously via the tail vein 24 hours after the induction of orchitis. Finally, both ciprofloxacin and P/E-selectin antibody were administered in TG 24 hours after the induction of orchitis for 14 days. At the end of treatment, 1 ml of serum sample was obtained to evaluate the ASA, P-selectin and E-selectin levels. In order to evaluate spermatogenesis (Johnsen score) and testicular injury (Cosentino score), both testes were extracted at the end of the 14th day.
In orchitis-induced groups (OG, ATG, PSEG, TG), ASA levels were significantly increased at the 14th day when compared to SG (p < 0.05). In TG, ASA levels were decreased when compared to AG. However, similar alteration in ASA levels was not detected in PSEG (p > 0.05). In OG and AG, P-selectin levels were decreased at the 14th day when compared to levels observed on 0 day (p < 0.05). E-selectin levels on 0 day showed that each group had higher levels of E-selectin when compared to CG (p > 0.05). There was no significant difference regarding E-selectin when compared to CG (p > 0.05). No significant differences regarding E-selectin levels were detected on the 0th and 14th days between AG and CG (p > 0.05). When the Cosentino and Johnsen scores were compared among groups, TG and PSEG has decreased scores of Cosentino than OG on the right testicle (p < 0.05). In contrast, an increased Johnsen score was detected in TG and PSEG when compared to OG (p < 0/05). No significant difference was detected for both Cosentino and Johnsen scores on the left testicle (p > 0.05). There was no difference with regard to the right and left testicular injury in TG. In P/E-blocked groups, decreased histopathological alterations were observed in the contralateral testis.
P/E-selectin blockage may reduce ASA production after orchitis when combined with antimicrobial treatment. P/E-selectin blockage not only has a protective effect on blood-testis barrier but also decreases the histopathological alterations in both the affected and contralateral testis. Histopathological parameters of spermatogenesis may also be prevented by P/E-selectin blockage in experimental orchitis.
本研究旨在评估 P/E-选择素阻断对实验性睾丸炎中抗精子抗体（ASA）发展和组织病理学改变的影响。
将 36 只体重为 100-150g 的 Wistar 白化雄性大鼠纳入研究。将大鼠分为 6 组（n=6），包括对照组（CG）、假手术组（SG）、睾丸炎组（OG）、抗菌治疗组（AG）、P/E-选择素阻断组（PESG）和抗菌和 P/E-选择素联合治疗组（TG）。在 CG 中，在手术前从尾静脉采集血清样本，然后提取双侧睾丸。在 SG 中，向睾丸实质内注射 1ml 生理盐水。通过向右侧睾丸注射 0.1ml106 cfu/ml 大肠杆菌（0:6 株）和 1ml 生理盐水来获得 OG。AG 在睾丸炎发生后 24 小时通过胃管每天两次给予环丙沙星（50mg/kg/天）。在 PESG 中，在睾丸炎发生后 24 小时通过尾静脉静脉内给予 P/E-选择素抗体（100μg）。最后，在睾丸炎发生后 24 小时，同时给予环丙沙星和 P/E-选择素抗体，治疗 14 天。在治疗结束时，从每个大鼠获得 1ml 血清样本，以评估 ASA、P-选择素和 E-选择素水平。为了评估精子发生（Johnsen 评分）和睾丸损伤（Cosentino 评分），在第 14 天结束时提取双侧睾丸。
在睾丸炎诱导组（OG、ATG、PESG、TG）中，与 SG 相比，第 14 天 ASA 水平显著升高（p<0.05）。在 TG 中，与 AG 相比，ASA 水平降低。然而，在 PESG 中未检测到类似的 ASA 水平变化（p>0.05）。在 OG 和 AG 中，与第 0 天相比，第 14 天 P-选择素水平降低（p<0.05）。第 0 天的 E-选择素水平表明，与 CG 相比，每组的 E-选择素水平都更高（p>0.05）。与 CG 相比，E-选择素水平没有显著差异（p>0.05）。在第 0 天和第 14 天，AG 与 CG 之间的 E-选择素水平没有显著差异（p>0.05）。在右侧睾丸中，与 OG 相比，TG 和 PESG 的 Cosentino 和 Johnsen 评分降低（p<0.05）。相比之下，与 OG 相比，TG 和 PESG 的 Johnsen 评分增加（p<0.05）。左侧睾丸的 Cosentino 和 Johnsen 评分无显著差异（p>0.05）。TG 对右侧和左侧睾丸损伤无差异。在 P/E 阻断组中，对侧睾丸的组织病理学改变减少。
在睾丸炎与抗菌治疗联合应用时，P/E-选择素阻断可能会减少 ASA 的产生。P/E-选择素阻断不仅对血睾屏障具有保护作用，而且还减少了受累和对侧睾丸的组织病理学改变。实验性睾丸炎中，P/E-选择素阻断还可能预防精子发生的组织病理学参数。
