Kass L
Stain Technol. 1985 Jul;60(4):233-7. doi: 10.3109/10520298509113917.
Using the acrylic textile dye Lycramine brilliant blue JL, mature and immature megakaryocytes from human bone marrow specimens stained metachromatically bright lavender. This coloration was not observed in other types of bone marrow cells. After digestion with either diastase or ribonuclease, subsequent staining of marrow specimens did not reveal a significant diminution of the intensity of staining of megakaryocytes. However, after incubation with hyaluronidase followed by staining with Lycramine brilliant blue JL, staining of megakaryocyte cytoplasm was either imperceptible or very pale blue. Accordingly, at least one of the substances responsible for the staining reaction is acid mucopolysaccharide in the cytoplasm of megakaryocytes. With further experience and comparison with established immunologic and cytochemical techniques, staining of megakaryocytes with Lycramine brilliant blue JL may be a useful addition to the cytochemistry of blood and bone marrow cells.
使用丙烯酸纺织染料Lycramine亮蓝JL对人骨髓标本中的成熟和未成熟巨核细胞进行染色后，巨核细胞呈现出异染性亮淡紫色。在其他类型的骨髓细胞中未观察到这种染色现象。用淀粉酶或核糖核酸酶消化后，随后对骨髓标本进行染色，未发现巨核细胞染色强度有明显减弱。然而，在用透明质酸酶孵育后再用Lycramine亮蓝JL染色，巨核细胞胞质的染色要么难以察觉，要么呈非常淡的蓝色。因此，至少一种负责染色反应的物质是巨核细胞胞质中的酸性粘多糖。随着经验的进一步积累以及与既定的免疫和细胞化学技术进行比较，用Lycramine亮蓝JL对巨核细胞进行染色可能会成为血液和骨髓细胞细胞化学的一项有用补充。
