Levy J, Rabinowitz R, Schlesinger M
Thymus. 1985;7(2):85-93.
Thymus cells of mice form rosettes with autologous and allogeneic erythrocytes. The nature of the thymus cell receptors which mediate the binding of erythrocytes is not known. The aim of the present study was to determine the effect of various antisera to T-cell specific antigens on the formation of rosettes by mouse thymus cells. In all strains of mice tested, the exposure of thymus cells to rabbit anti-mouse brain serum (RABR) was found to inhibit autorosette formation. Similarly, monoclonal Thy-1 antibodies inhibited the formation of autorosettes by thymus cells in all strains tested. Monoclonal antibodies against Lyt-1, Lyt-2, and TL determinants had no such effect. Monoclonal Thy-1 antibodies inhibited the formation of rosettes with allogeneic erythrocytes only when the thymus contained the Lyt-2.2 allele (BALB/c, C57BL), but not when it contained the Lyt-2.1 allele (AKR/J, C3H, DBA/2). These results indicate that Thy-1 determinants on thymus cells are involved in the recognition of 'self' structures, shared by erythrocytes of all strains of mice. Lyt-2 determinants may play a role in the recognition of 'non-self', allogeneic determinants, but the thymus cell surface structures encoded by the two Lyt-2 alleles may differ in their affinity to allogeneic erythrocytes.
小鼠胸腺细胞可与自身及同种异体红细胞形成玫瑰花结。介导红细胞结合的胸腺细胞受体的性质尚不清楚。本研究的目的是确定各种针对T细胞特异性抗原的抗血清对小鼠胸腺细胞形成玫瑰花结的影响。在所有测试的小鼠品系中，发现胸腺细胞暴露于兔抗小鼠脑血清（RABR）会抑制自身玫瑰花结的形成。同样，单克隆Thy-1抗体在所有测试品系中均抑制胸腺细胞形成自身玫瑰花结。针对Lyt-1、Lyt-2和TL决定簇的单克隆抗体没有这种作用。单克隆Thy-1抗体仅在胸腺含有Lyt-2.2等位基因（BALB/c、C57BL）时抑制与同种异体红细胞形成玫瑰花结，而当胸腺含有Lyt-2.1等位基因（AKR/J、C3H、DBA/2）时则无此作用。这些结果表明，胸腺细胞上的Thy-1决定簇参与了对所有小鼠品系红细胞共有的“自身”结构的识别。Lyt-2决定簇可能在识别“非自身”同种异体决定簇中起作用，但由两个Lyt-2等位基因编码的胸腺细胞表面结构对同种异体红细胞的亲和力可能不同。
