Department of Clinical Research, Oriental Medicine Research Center, Kitasato University, Japan.
J Pharmacol Sci. 2013;123(2):176-84. doi: 10.1254/jphs.13121fp. Epub 2013 Oct 4.
The conventional method for the real-time assessment of murine colitis requires a large number of animals. The (13)C-butyrate breath test could be useful for evaluating disease activity and the amelioration of human ulcerative colitis non-invasively. The purpose of this study was to investigate whether this test can be used to assess the phase of inflammation in murine colitis. We investigated the excretion of (13)CO2 measured by the (13)C-butyrate breath test after rectal instillation of butyrate in the DSS colitis model. The colon length, MPO activity, and histological damage were analyzed as parameters. The efficacy of salicylazosulfa-pyridine (SASP) on (13)CO2 excretion was also studied. The (13)CO2 excretion curves in the 0.5% DSS- and 0.75% DSS-treated groups were significantly lower than those in the normal group (P < 0.01, P < 0.01). Good correlation between the results of the breath test and the inflammation parameters was observed. The (13)CO2 excretion curve in DSS murine colitis after the administration of SASP was significantly higher than in the normal group (P < 0.01). The (13)C-butyrate breath test can be used to evaluate the inflammatory phase of DSS murine colitis, and it may be a new non-invasive method for assessing murine colitis.
传统的实时评估小鼠结肠炎的方法需要大量的动物。（13）C-丁酸呼气试验可用于非侵入性地评估人类溃疡性结肠炎的疾病活动度和改善情况。本研究旨在探讨该试验是否可用于评估小鼠结肠炎的炎症阶段。我们研究了通过直肠给予丁酸钠后，在 DSS 结肠炎模型中（13）CO2 的排泄情况，以（13）C-丁酸呼气试验进行测量。分析了结肠长度、MPO 活性和组织学损伤等参数。还研究了柳氮磺胺吡啶（SASP）对（13）CO2 排泄的疗效。在 0.5% DSS 和 0.75% DSS 处理组中，（13）CO2 排泄曲线明显低于正常组（P < 0.01，P < 0.01）。呼气试验结果与炎症参数之间存在良好的相关性。SASP 给药后 DSS 诱导的小鼠结肠炎中的（13）CO2 排泄曲线明显高于正常组（P < 0.01）。（13）C-丁酸呼气试验可用于评估 DSS 诱导的小鼠结肠炎的炎症阶段，它可能是一种评估小鼠结肠炎的新的非侵入性方法。
