Kawai-Yamada Maki, Nagano Minoru, Kakimoto Masayuki, Uchimiya Hirofumi
Graduate School of Science and Engineering, Saitama University, 255 Shimo-okubo, Sakura-ku, Saitama, 338-8570, Japan,
Planta. 2014 Jan;239(1):39-46. doi: 10.1007/s00425-013-1966-1. Epub 2013 Oct 6.
Arabidopsis cell growth defect factor-1 (Cdf1 in yeast, At5g23040) was originally isolated as a cell growth suppressor of yeast from genetic screening. To investigate the in vivo role of Cdf1 in plants, a T-DNA insertion line was analyzed. A homozygous T-DNA insertion mutant (cdf1/cdf1) was embryo lethal and showed arrested embryogenesis at the globular stage. The Cdf1 protein, when fused with green fluorescent protein, was localized to the plastid in stomatal guard cells and mesophyll cells. A promoter-β-glucuronidase assay found expression of Cdf1 in the early heart stage of embryogenesis, suggesting that Cdf1 was essential for Arabidopsis embryogenesis during the transition of the embryo from the globular to heart stage.
拟南芥细胞生长缺陷因子-1（酵母中的Cdf1，At5g23040）最初是通过遗传筛选从酵母中作为细胞生长抑制因子分离出来的。为了研究Cdf1在植物体内的作用，对一个T-DNA插入系进行了分析。一个纯合的T-DNA插入突变体（cdf1/cdf1）胚胎致死，并在球形期显示胚胎发育停滞。当Cdf1蛋白与绿色荧光蛋白融合时，定位于气孔保卫细胞和叶肉细胞的质体中。启动子-β-葡萄糖醛酸酶分析发现Cdf1在胚胎发育的早期心形期表达，这表明Cdf1在胚胎从球形期向心形期转变过程中对拟南芥胚胎发育至关重要。
