Chen Jin, Chen Kejie, Yuan Shibin, Peng Xi, Fang Jing, Wang Fengyuan, Cui Hengmin, Chen Zhengli, Yuan Jingxin, Geng Yi
Department of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, People's Republic of China.
Institute of Rare Animals and Plants, College of Life Sciences, China West Normal University, Nanchong, Sichuan, People's Republic of China.
Toxicol Ind Health. 2016 Feb;32(2):278-84. doi: 10.1177/0748233713500819. Epub 2013 Oct 4.
The purpose of the present study was to investigate the oxidative damage and apoptosis induced by aflatoxin B1 (AFB1) in spleen of broilers. A total of 200 one-day-old avian male broilers were randomly divided into 4 equal groups of 50 each and were fed for 21 days as follows: a control diet and three AFB1 diets containing 0.15, 0.3, and 0.6 mg AFB1/kg diet. Consumption of AFB1 diets induced oxidative stress in the spleen of chicken as evidenced by reduced glutathione peroxidase, glutathione reductase, and catalase activities, decreased glutathione contents, and increased malondialdehyde contents in explaining the pathogenesis. Flow cytometer method and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling assay revealed that the apoptotic splenocytes were increased in AFB1 groups. The results suggest that AFB1 induced excessive apoptosis of splenic lymphocytes, which is correlated with increased oxidative stress. The present results may be helpful for explaining the pathogenesis of AFB1-induced immunosuppression.
本研究的目的是调查黄曲霉毒素B1（AFB1）对肉鸡脾脏造成的氧化损伤和细胞凋亡。总共200只1日龄雄性肉鸡被随机分为4组，每组50只，按以下方式饲养21天：一组为对照日粮组，另外三组为分别含有0.15、0.3和0.6毫克AFB1/千克日粮的AFB1日粮组。食用AFB1日粮会导致鸡脾脏产生氧化应激，表现为谷胱甘肽过氧化物酶、谷胱甘肽还原酶和过氧化氢酶活性降低，谷胱甘肽含量减少，丙二醛含量增加，这些变化解释了其发病机制。流式细胞仪检测法和末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记法显示，AFB1组凋亡的脾细胞增多。结果表明，AFB1诱导脾淋巴细胞过度凋亡，这与氧化应激增加有关。本研究结果可能有助于解释AFB1诱导免疫抑制的发病机制。
