分离的大鼠胃黏膜细胞的组胺含量与释放

Histamine content and release of isolated rat gastric mucosal cells.

作者信息

Ruoff H J, Reutter K, Schepp W

出版信息

Agents Actions. 1985 Apr;16(3-4):202-4. doi: 10.1007/BF01983139.

DOI:10.1007/BF01983139

Abstract

Enzymatically dispersed rat gastric cells were subdivided (Percoll) in fractions (F1, F2, F3) with different number of parietal cells (PC) and the intracellular histamine content was estimated (ng/10(6) cells): F1 (9% PC): 17 +/- 4 (SEM), F2 (26% PC): 80 +/- 9 and F3 (77% PC): 134 +/- 15. Histidinedecarboxylase showed the same pattern of distribution, F1 low, F2 medium, F3 high activity. Incubated F3 cells constantly released histamine (19 +/- 3 ng/10(6) cells/h), a process which could be stimulated by carbachol, forskolin and hexoprenaline. The data suggest: rat gastric histaminocytes copurify with PC, background stimulation by histamine should be considered in isolated cell systems, vagal stimulation may release histamine within the gastric mucosa.

摘要

将酶解分散的大鼠胃细胞（通过Percoll）分成具有不同壁细胞数量（PC）的级分（F1、F2、F3），并估计细胞内组胺含量（ng/10⁶个细胞）：F1（9% PC）：17±4（标准误），F2（26% PC）：80±9，F3（77% PC）：134±15。组氨酸脱羧酶呈现相同的分布模式，F1活性低，F2活性中等，F3活性高。孵育的F3细胞持续释放组胺（19±3 ng/10⁶个细胞/小时），这一过程可被卡巴胆碱、福斯可林和海索那林刺激。数据表明：大鼠胃组胺细胞与壁细胞共纯化，在分离的细胞系统中应考虑组胺的背景刺激，迷走神经刺激可能在胃黏膜内释放组胺。