Gottlieb M H, Steer C J, Steven A C, Chrambach A
Anal Biochem. 1985 Jun;147(2):353-63. doi: 10.1016/0003-2697(85)90282-9.
Agarose gel electrophoresis was found to be applicable to the physical characterization of clathrin-coated vesicles from bovine brain and rat liver. The vesicles from brain are smaller and, at pH 6.4, more highly charged than those from liver. Using a standard curve for spherical viruses, the mean radii of the brain vesicles averaged 40 +/- 3 nm for two preparations, and those of liver vesicles 53 +/- 5 nm. When the comparison between the two species is made within the same experiment, the size difference between rat liver-derived and bovine brain-derived clathrin-coated vesicles is significant at the 95% confidence level. The sizes are compatible with electron microscopy measurements. The mobilities (uncorrected for electroendosmosis), extrapolated to 0% agarose on the Ferguson plot, mu'0 (cm2/sec/V), were 0.76 +/- 0.04 for brain vesicles and 0.61 +/- 0.09 for liver vesicles. These are measures of the average surface net charge of the vesicles. The higher net charge of brain vesicles, compared to liver vesicles, is significant at the 95% confidence level whether the two species are compared intra- or interexperimentally. Charge differences between two different preparations from the same organ were significant at the 95% confidence level, while size differences were not.
琼脂糖凝胶电泳被发现可用于对来自牛脑和大鼠肝脏的网格蛋白包被囊泡进行物理表征。来自大脑的囊泡比来自肝脏的囊泡更小，并且在pH 6.4时，带电量更高。使用球形病毒的标准曲线，两种制备物中脑囊泡的平均半径平均为40±3nm，肝囊泡的平均半径为53±5nm。当在同一实验中对这两个物种进行比较时，大鼠肝脏来源和牛脑来源的网格蛋白包被囊泡之间的大小差异在95%置信水平上具有显著性。这些大小与电子显微镜测量结果相符。在弗格森图上外推至0%琼脂糖时的迁移率（未校正电渗），μ'0（cm2/sec/V），脑囊泡为0.76±0.04，肝囊泡为0.61±0.09。这些是囊泡平均表面净电荷的度量。无论是在实验内还是实验间比较这两个物种，与肝囊泡相比，脑囊泡的较高净电荷在95%置信水平上具有显著性。来自同一器官的两种不同制备物之间的电荷差异在以95%置信水平具有显著性，而大小差异则不然。
