黑腹果蝇琥珀酰辅酶A：3-酮酸辅酶A转移酶的结构
Structure of succinyl-CoA:3-ketoacid CoA transferase from Drosophila melanogaster.
作者信息
Zhang Min, Xu Han-Yang, Wang Yi-Cui, Shi Zhu-Bing, Zhang Nan-Nan
机构信息
School of Life Science, Anhui University, Hefei 230026, People's Republic of China.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Oct;69(Pt 10):1089-93. doi: 10.1107/S1744309113024986. Epub 2013 Sep 28.
Abstract
Succinyl-CoA:3-ketoacid CoA transferase (SCOT) plays a crucial role in ketone-body metabolism. SCOT from Drosophila melanogaster (DmSCOT) was purified and crystallized. The crystal structure of DmSCOT was determined at 2.64 Å resolution and belonged to space group P212121, with unit-cell parameters a=76.638, b=101.921, c=122.457 Å, α=β=γ=90°. Sequence alignment and structural analysis identified DmSCOT as a class I CoA transferase. Compared with Acetobacter aceti succinyl-CoA:acetate CoA transferase, DmSCOT has a different substrate-binding pocket, which may explain the difference in their substrate specificities.
摘要
琥珀酰辅酶A：3-酮酸辅酶A转移酶（SCOT）在酮体代谢中起关键作用。对黑腹果蝇的SCOT（DmSCOT）进行了纯化和结晶。DmSCOT的晶体结构在2.64 Å分辨率下测定，属于空间群P212121，晶胞参数a = 76.638、b = 101.921、c = 122.457 Å，α = β = γ = 90°。序列比对和结构分析确定DmSCOT为I类辅酶A转移酶。与醋酸醋杆菌琥珀酰辅酶A：乙酸辅酶A转移酶相比，DmSCOT具有不同的底物结合口袋，这可能解释了它们底物特异性的差异。
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