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[紫外光下及用焦宁染色检测的间期细胞核中染色质结构一致性的细胞光度分析]

[Cytophotometric analysis of the chromatin structural conformity in interphase nuclei detected in UV light and by gallocyanine staining].

作者信息

Zhukotskiĭ A V, Shchegolev A I, Butusova N N, Nemirovskiĭ L E, Kogan E M

出版信息

Biull Eksp Biol Med. 1985 Jun;99(6):726-7.

PMID:2410060
Abstract

Geometric and optical parameters of chromatin of hepatocyte nuclei have been examined before (UV, lambda = 265 nm) and after gallocyanine staining. Quantitative parameters of the chromatin structure in the same nuclei measured in situ by a scanning microscope-photometer (step size 0.125 micron) before and after staining were equal. Tinctorial properties of chromatin granules (condensed part of the nuclear material) and its diffuse part were different. It is suggested that the difference between granules and the nongranular part of chromatin is not only of optical but also of chemical nature.

摘要

在使用镓氰宁染色之前(紫外线,波长=265纳米)和之后,对肝细胞核染色质的几何和光学参数进行了检测。用扫描显微镜光度计(步长0.125微米)在原位测量同一细胞核染色前后染色质结构的定量参数是相等的。染色质颗粒(核物质的浓缩部分)及其弥散部分的染色特性是不同的。有人提出,染色质颗粒与非颗粒部分之间的差异不仅具有光学性质,而且具有化学性质。

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