Wang Hong-Wei, Zhu Hong, Liang Xue-Fei, Du Wei, Dai Chuan-Chao
Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, Jiangsu Province, China,
Biotechnol Lett. 2014 Jan;36(1):167-73. doi: 10.1007/s10529-013-1347-7. Epub 2013 Oct 8.
A novel laccase (LACB3) from the endophytic fungus, Phomopsis liquidambari, was cloned and its potential to promote peanut growth was evaluated. The full-length cDNA is 1,731 bp, encoding a mature protein of 556 amino acids with a molecular mass of 60.1 kDa. Using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate), LACB3 exhibited a K m and k cat of 85 μM and 92.7 s(-1), respectively. The enzyme had maximal activity at pH 2.5 and 50 °C and retained 50 % of its activity after 20 h at 50 °C. When LACB3 was applied to soil, the peanut biomass was increased by 12 %, and the content of vanillic acid, coumaric acid and 4-hydroxybenzoic acid in soil were decreased by 21, 27 and 40 %, respectively. These results suggest substantial potential for the use of P. liquidambari or its laccase in agriculture.
从内生真菌枫香拟茎点霉中克隆出一种新型漆酶（LACB3），并评估了其促进花生生长的潜力。该全长cDNA为1731 bp，编码一个由556个氨基酸组成的成熟蛋白，分子量为60.1 kDa。使用2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸），LACB3的K m和k cat分别为85 μM和92.7 s(-1)。该酶在pH 2.5和50 °C时具有最大活性，在50 °C下20小时后仍保留其50%的活性。当将LACB3施用于土壤时，花生生物量增加了12%，土壤中香草酸、香豆酸和4-羟基苯甲酸的含量分别降低了21%、27%和40%。这些结果表明，枫香拟茎点霉或其漆酶在农业中有很大的应用潜力。
