Denk H, Weybora W, Ratschek M, Sohar R, Franke W W
Differentiation. 1985;29(1):88-97. doi: 10.1111/j.1432-0436.1985.tb00297.x.
The distribution of cytokeratins, desmosomal-plaque proteins (desmoplakins), and vimentin in nephroblastoma tissue was studied by immunofluorescence microscopy using specific antibodies. In undifferentiated blastema cells, desmosomes, as revealed by antibodies to desmoplakins, preceded the advent of significant amounts of cytokeratins, indicating that desmosomes are early and sensitive markers of epithelial differentiation. Cytokeratin-positive tumor cells were seen in the following distribution patterns: groups of loosely arranged and scattered cells containing only scant cytokeratin fibrils surrounded by negative stroma cells; focal accumulation of cytokeratin-positive cells with cytokeratin-specific cytoplasmic fibril meshwork staining; rosettes of cytokeratin-positive cells without formation of distinct lumina, showing concentration of cytokeratin staining in the center; tubules with distinct lumina made up of cytokeratin-positive cells, with cytokeratin staining concentrated in the subapical cell portions. In cytokeratin-positive cells, the numbers of desmoplakin-positive dots were generally increased; in well-formed tubules, enrichment of desmoplakin-positive spots, corresponding to the subapical skeletal disks, was most conspicuous. Vimentin was demonstrated in stromal areas, but also in blastema cells showing coexpression of desmosomes and vimentin filaments. Moreover, in certain blastema cells, an overlap of cytokeratin and vimentin immunostaining was observed. Epithelial cells of nephroblastoma tubules did not react with vimentin antibodies. Our results show that the appearance of desmosomal plaques, as demonstrated by antibodies to desmoplakins, may be a very early feature of epithelial differentiation, and they also emphasize the value of antibodies to desmoplakins in tumor cell typing and diagnosis.
利用特异性抗体，通过免疫荧光显微镜研究了肾母细胞瘤组织中细胞角蛋白、桥粒斑蛋白（桥粒芯蛋白）和波形蛋白的分布。在未分化的胚基细胞中，桥粒芯蛋白抗体所显示的桥粒，先于大量细胞角蛋白的出现，这表明桥粒是上皮分化的早期且敏感的标志物。细胞角蛋白阳性的肿瘤细胞呈现以下分布模式：仅含有少量细胞角蛋白原纤维的松散排列和散在细胞群，被阴性基质细胞包围；细胞角蛋白阳性细胞的局灶性聚集，伴有细胞角蛋白特异性的细胞质原纤维网状染色；细胞角蛋白阳性细胞形成的玫瑰花结，无明显管腔形成，细胞角蛋白染色集中在中心；由细胞角蛋白阳性细胞组成的有明显管腔的小管，细胞角蛋白染色集中在细胞顶端下部分。在细胞角蛋白阳性细胞中，桥粒芯蛋白阳性点的数量通常增加；在形成良好的小管中，与顶端下骨架盘相对应的桥粒芯蛋白阳性斑点的富集最为明显。波形蛋白在基质区域中被证实存在，也在显示桥粒和波形蛋白丝共表达的胚基细胞中存在。此外，在某些胚基细胞中，观察到细胞角蛋白和波形蛋白免疫染色有重叠。肾母细胞瘤小管的上皮细胞不与波形蛋白抗体发生反应。我们的结果表明，桥粒芯蛋白抗体所证实的桥粒斑的出现，可能是上皮分化的一个非常早期的特征，并且它们还强调了桥粒芯蛋白抗体在肿瘤细胞分型和诊断中的价值。
