Tu Xue-ping, Wang Nian, Hu Ke, Xianyu Yun-yan, Cao Xia, Chen Hong-bin, Kang Jing
Department of Respiratory Disease, Renmin Hospital of Wuhan University, Wuhan 430060, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2013 Jun;36(6):437-40.
To explore the impact of intermittent hypoxia on long-term facilitation (LTF) of hypoglossal nerve discharge.
Twelve adult SD rats were divided into the experimental group (CIH group, n = 6) and the control group (normoxia group, n = 6) by the random number table. The rats in the CIH group were fed in the intermittent hypoxia animal chambers, while the control group was placed in the normoxia animal chambers for 8 h per day (from 8:00 AM to 4:00 PM) for 4 consecutive weeks. After that, 5 min×3 stimulations of acute intermittent hypoxia (AIH) were administered and the hypoglossal never signals were recorded before and after AIH.
The baseline frequency and average peak amplitude of hypoglossal nerve discharge in the CIH experimental group were significantly greater than those in the control group. The discharge frequency in the CIH and the control groups was (73 ± 13) Hz, and (58 ± 11) Hz, respectively(P < 0.05); and the discharge amplitude in the 2 groups was (4.6 ± 1.1) µV, and (3.3 ± 0.7) µV, respectively(P < 0.05). After intervention with AIH, the frequency and the average peak amplitude of the hypoglossal nerve discharge in the experimental and the control groups were significantly increased(all P < 0.05). The increased discharge lasted more than 1 h and this typical phenomenon was referred to as LTF. In the CIH group, the discharge frequency before and after exposure to AIH was (68 ± 16) Hz and (133 ± 20) Hz, respectively; and the discharge amplitude was (4.6 ± 1.1) µV and (8.9 ± 1.4) µV, respectively. In the control group, the discharge frequency before and after AIH was (59 ± 12) Hz and (102 ± 16) Hz, respectively; and the discharge amplitude was (3.3 ± 0.7) µV and (4.5 ± 0.7) µV, respectively(P < 0.05). After AIH stimulation, the enhanced respiratory intensity of rats in CIH group was much higher than that in the control group [(408 ± 149)% vs (242 ± 31)%, P < 0.05].
Both AIH and CIH can induce LTF of the hypoglossal nerve discharge, while the induction of LTF by AIH can be strengthened by CIH.
探讨间歇性低氧对舌下神经放电长时程易化（LTF）的影响。
将12只成年SD大鼠通过随机数字表法分为实验组（慢性间歇性低氧组，CIH组，n = 6）和对照组（常氧组，n = 6）。CIH组大鼠置于间歇性低氧动物舱内饲养，而对照组置于常氧动物舱内，每天8小时（上午8:00至下午4:00），连续4周。之后，给予5分钟×3次的急性间歇性低氧（AIH）刺激，并记录AIH前后的舌下神经信号。
CIH实验组舌下神经放电的基线频率和平均峰值幅度显著高于对照组。CIH组和对照组的放电频率分别为（73±13）Hz和（58±11）Hz（P<0.05）；两组的放电幅度分别为（4.6±1.1）μV和（3.3±0.7）μV（P<0.05）。经AIH干预后，实验组和对照组舌下神经放电的频率和平均峰值幅度均显著增加（均P<0.05）。放电增加持续超过一小时，这种典型现象被称为LTF。在CIH组中，暴露于AIH前后的放电频率分别为（68±16）Hz和（133±20）Hz；放电幅度分别为（4.6±1.1）μV和（8.9±1.4）μV。在对照组中，AIH前后的放电频率分别为（59±12）Hz和（102±16）Hz；放电幅度分别为（3.3±0.7）μV和（4.5±0.7）μV（P<0.05）。AIH刺激后，CIH组大鼠呼吸强度的增强幅度远高于对照组[（408±149）%对（242±31）%，P<0.05]。
AIH和CIH均可诱导舌下神经放电的LTF，而CIH可增强AIH对LTF的诱导作用。
