Bürger H, Steuler H, Scholtissek C
J Gen Virol. 1985 Aug;66 ( Pt 8):1679-86. doi: 10.1099/0022-1317-66-8-1679.
A temperature-sensitive mutant (ts 1/9) obtained by undiluted passage of fowl plague virus (FPV) at 33 degrees C carried a strong ts defect in RNA segment 6 [neuraminidase (NA) gene] and a weak ts defect in RNA segment 8 [non-structural (NS) protein Although the viral proteins have normal migration rates, the NS gene migrated during polyacrylamide gel electrophoresis (PAGE) significantly faster than the NS gene of wild-type FPV, even after denaturation by glyoxal. Despite this observation, the NS gene of ts 1/9 did not carry a deletion as shown by sequence determination. There were only five base replacements which resulted in three changes in amino acids. Three of the base replacements led to a more compact secondary structure of RNA segment 8, which seems to be responsible for the faster migration rate during PAGE and which seems to resist, at least partially, the treatment with glyoxal.
通过在33℃下对禽痘病毒（FPV）进行未稀释传代获得的温度敏感突变体（ts 1/9），在RNA片段6[神经氨酸酶（NA）基因]中存在强烈的温度敏感缺陷，在RNA片段8[非结构（NS）蛋白]中存在较弱的温度敏感缺陷。虽然病毒蛋白具有正常的迁移率，但即使在用乙二醛变性后，NS基因在聚丙烯酰胺凝胶电泳（PAGE）过程中的迁移速度也明显快于野生型FPV的NS基因。尽管有此观察结果，但序列测定表明ts 1/9的NS基因没有缺失。只有五个碱基替换，导致三个氨基酸变化。其中三个碱基替换导致RNA片段8的二级结构更紧密，这似乎是PAGE过程中迁移速度更快的原因，并且似乎至少部分地抵抗乙二醛处理。
