Krivonosov S K, Ivkova L A
Ontogenez. 1985 May-Jun;16(3):265-9.
Molecular weight of PSP, determined by gel-filtration on Sephadex G-200, is 130 000 +/- 4 000 D and, according to the Laemmli method with SDS, 85 000 +/- 2 000 D. The protein was detected in the zone of beta 1-globulins, its relative electrophoretic mobility is 0.45 +/- 0,2. PSP is heterogeneous by electric charge; at electrofocusing this protein was revealed in the following isoelectric points (pI); 5.85, 6.11, and 6.57. PSP was precipitated with ammonium sulfate (20-50% of saturation). Iron, carbohydrates, lipids were not found histochemically in PSP and it did not manifest the esterase and phosphatase activity.
通过在葡聚糖凝胶G - 200上进行凝胶过滤测定，PSP的分子量为130000±4000道尔顿，而根据使用SDS的Laemmli方法测定，其分子量为85000±2000道尔顿。该蛋白质在β1 -球蛋白区域被检测到，其相对电泳迁移率为0.45±0.02。PSP在电荷方面具有异质性；在等电聚焦时，该蛋白质在以下等电点（pI）被发现：5.85、6.11和6.57。PSP可被硫酸铵沉淀（饱和度为20 - 50%）。在PSP中未通过组织化学方法检测到铁、碳水化合物、脂质，并且它也不表现出酯酶和磷酸酶活性。
