Coleman A W, Goff L J
Stain Technol. 1985 May;60(3):145-54. doi: 10.3109/10520298509113905.
The two DNA-specific fluorochromes DAPI and mithramycin have been found to be extremely useful dyes in studies of pollen development and growth. Both fluorochromes stain nuclei brilliantly either in fixed or in living tricellular and bicellular angiosperm pollen, thereby permitting rapid scanning for pollen abnormalities and easy observation of nuclear details. These water soluble dyes can be incorporated into the germination medium for studies of pollen germination in vitro, facilitating observation of the movement of generative, sperm and tube nuclei during pollen growth. In fixed pollen, the fluorochromes bind quantitatively with DNA and thus may be used to quantitate ploidy changes and to study cell cycles during pollen development, germination and fertilization.
已发现两种DNA特异性荧光染料——4',6-二脒基-2-苯基吲哚（DAPI）和光神霉素，在花粉发育和生长研究中是极为有用的染料。这两种荧光染料在固定的或活的三细胞和二细胞被子植物花粉中都能出色地对细胞核进行染色，从而能够快速扫描花粉异常情况并轻松观察细胞核细节。这些水溶性染料可掺入萌发培养基中用于体外花粉萌发研究，便于观察花粉生长过程中生殖核、精核和管核的移动。在固定的花粉中，荧光染料与DNA定量结合，因此可用于定量倍性变化以及研究花粉发育、萌发和受精过程中的细胞周期。
