非消融性高射频治疗对兔真皮细胞外基质的中长期影响。

Mid-long term effect of non-ablative high radiofrequency therapy on the rabbit dermal extracellular matrix.

作者信息

Choi S, Shin J H, Nam S W, Jang H, Tao T, Kwak H W, Jin K H, Lee G J, Park H K

出版信息

Annu Int Conf IEEE Eng Med Biol Soc. 2013;2013:3761-4. doi: 10.1109/EMBC.2013.6610362.

DOI:10.1109/EMBC.2013.6610362

Abstract

This study quantitatively investigated the postoperative effects of radiofrequency (RF) application on the normal dermal extracellular matrix (ECM) of in vivo rabbits. Postoperative effects were evaluated by histology and atomic force microscopy analysis of dermal tissues treated using three RF energy levels (10 ~ 30 W) and either a single- or multiple-pass procedure. Progressive changes in the morphology of rabbit dermal ECMs were investigated over a 30-day postoperartive period. All RF-treated groups, except for the low energy group (10 W), displayed more prominent inflammatory responses compared to the control. This inflammatory reaction was more prominent a day after application. Dermal tissues 30 days after RF application exhibited prominent myofibroblast activity associated with ECM contractile activity during wound healing in addition to chronic inflammation. A decrease in the morphology of dermal ECMs after RF application continued until seven days postoperatively. The ECM diameter increased to near baseline at 30 days postoperatively. Low energy and multi-pass applications resulted in greater collagen fibril contraction and recovery at the ultra-structural level at 30 days postoperatively than did a single high energy application.

摘要

本研究定量调查了射频（RF）应用于体内兔正常真皮细胞外基质（ECM）后的术后效果。通过组织学和原子力显微镜分析，对使用三种射频能量水平（10~30W）以及单次或多次治疗程序处理的真皮组织的术后效果进行评估。在术后30天的时间段内，研究了兔真皮ECM形态的渐进性变化。除低能量组（10W）外，所有射频治疗组与对照组相比均表现出更明显的炎症反应。这种炎症反应在治疗后一天更为明显。射频治疗30天后的真皮组织除了慢性炎症外，在伤口愈合过程中还表现出与ECM收缩活性相关的显著成肌纤维细胞活性。射频应用后真皮ECM形态的减小持续至术后7天。术后30天，ECM直径增加至接近基线水平。与单次高能量应用相比，低能量和多次应用在术后30天的超微结构水平上导致更大的胶原纤维收缩和恢复。