Ogawa Tomohisa, Matsunaga Nirai, Inomata Saori, Tanaka Masato, Futai Nobuyuki
Annu Int Conf IEEE Eng Med Biol Soc. 2013;2013:5521-4. doi: 10.1109/EMBC.2013.6610800.
We have developed a cast microfluidic chip that contains a thin (~2 µm wide) microchannel that is smoothly connected to thick microfluidics. The thin line features having high aspect ratio for a low-cost photolithography in which an emulsion photomask was used (1:1 ~ 1:3) were fabricated by exposing SU-8 photoresist to diffused 185 nm UV light emitted by a low-cost ozone lamp from the backside of the substrate to ensure sufficient crosslinking of small regions of the SU-8 photoresist. An H-shaped microfluidic configuration was used, in which the thin channel maintained constant diffusion fronts beyond purely static diffusion. We also demonstrated the long-term effects of a gradient of nerve growth factor on axon elongation by primary neurons cultured in the micro channel.
我们开发了一种铸型微流控芯片，该芯片包含一条窄的（约2微米宽）微通道，该微通道与厚壁微流体装置平滑连接。通过使用乳液光掩膜（1:1至1:3）进行低成本光刻来制造具有高纵横比的细线特征，具体做法是从基板背面用低成本臭氧灯发出的漫射185纳米紫外线照射SU-8光刻胶，以确保SU-8光刻胶小区域的充分交联。采用了H形微流控结构，其中细通道在纯静态扩散之外保持恒定的扩散前沿。我们还通过在微通道中培养的原代神经元，证明了神经生长因子梯度对轴突伸长的长期影响。
