Department of Respiratory and Critical Care Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Chin Med J (Engl). 2013;126(19):3608-15.
Cigarette smoke induces an acute but persisting inflammation in peripheral blood and airway in chronic obstructive pulmonary disease (COPD), and CD8(+) Tc-lymphocytes are considered as a key role in this process. We aimed to investigate the Tc-lymphocytes immunodeviation in system and local airway of COPD patients and changes of the immunodeviation after short-term smoking cessation.
Peripheral blood (PB) and bronchoalveolar lavage fluid (BALF) were collected from 42 patients (14 COPD patients, 16 smokers with normal lung function and 12 nonsmokers), while PB and induced sputum (IS) were obtained from other 19 patients (10 quitting smokers and 9 continuing smokers) at baseline and follow-up respectively of 4-week smoking cessation. Percentages of CD8(+) Tc-lymphocytes (%CD3(+)) and Tc1/Tc2 ratios were measured by flow cytometry.
Percentages of CD8(+) Tc-lymphocytes were higher in COPD patients than those in smokers and nonsmokers in both PB and BALF. Tc1/Tc2 ratio in PB and in BALF from COPD patients was greater than that from smokers and nonsmokers and negatively correlated with FEV1 %pre. When comparing the ratios between PB and BALF, significantly positive correlation was found in COPD patients. Furthermore, after 4-week smoking cessation, percentages of CD8(+) Tc-lymphocytes in PB and IS in quitting smokers were decreased compared to that in baseline and continuing smokers, whereas Tc1/Tc2 ratios were not influenced.
CD8(+) Tc1-trend immunodeviation profiles occurred in both system and local airway of COPD patients. This exceptional immunodeviation could not be relieved by short-term smoking cessation.
香烟烟雾在慢性阻塞性肺疾病（COPD）患者的外周血和气道中引发急性但持续的炎症，CD8+Tc 淋巴细胞被认为在此过程中起关键作用。我们旨在研究 COPD 患者系统和局部气道中 Tc 淋巴细胞的免疫偏离以及短期戒烟后免疫偏离的变化。
从 42 名患者（14 名 COPD 患者、16 名肺功能正常的吸烟者和 12 名不吸烟者）中采集外周血（PB）和支气管肺泡灌洗液（BALF），从另外 19 名患者（10 名戒烟者和 9 名继续吸烟者）中采集 PB 和诱导痰（IS），分别在基线和 4 周戒烟随访时。通过流式细胞术测量 CD3+CD8+Tc 淋巴细胞的百分比（%CD3+）和 Tc1/Tc2 比值。
COPD 患者的 PB 和 BALF 中 CD8+Tc 淋巴细胞的百分比均高于吸烟者和不吸烟者。COPD 患者的 PB 和 BALF 中的 Tc1/Tc2 比值大于吸烟者和不吸烟者，与 FEV1%pre 呈负相关。当比较 PB 和 BALF 之间的比值时，COPD 患者的比值之间存在显著的正相关。此外，在 4 周戒烟后，戒烟者的 PB 和 IS 中的 CD8+Tc 淋巴细胞百分比与基线和继续吸烟者相比降低，而 Tc1/Tc2 比值未受影响。
COPD 患者的系统和局部气道中存在 CD8+Tc1 趋势的免疫偏离。这种特殊的免疫偏离不能通过短期戒烟来缓解。
