Department of Gastrointestinal Surgery, the First Affiliated Hospital, Hebei North University, Zhangjiakou, Hebei 075000, China.
Chin Med J (Engl). 2013;126(19):3739-44.
Andrographolide has been shown to have anticancer activity on diverse cancer cell lines representing different types of human cancers. The aim of this research was to investigate the anticancer and apoptotic effects of andrographolide on the BGC-823 human gastric cancer cell line.
Cell proliferation and IC50 were evaluated using MTT assay, cell-cycle analysis with flow cytometry apoptotic effects with Annexin-V/propidium iodide double-staining assay, and morphologic structure with transmission electron microscopy. Immunohistochemistry and reverse-transcription PCR was used to analyze Bcl-2, Bax, and caspase-3 expressions.
Andrographolide showed a time- and concentration-dependent inhibitory effects on BGC-823 cell growth. Compared to controls, the number of cells in the G0-G1-phase increased significantly, S and G2-M-phase cells decreased after 48 hours of treatment with andrographolide, and both early and late apoptotic rates increased significantly compared to the controls, all in a concentration-dependent manner. Bax and caspase-3 expressions were markedly increased, and Bcl-2 expression was decreased.
Andrographolide inhibits BGC-823 cell growth and induces BGC-823 cell apoptosis by up-regulating Bax and caspase-3 expressions and down-regulating Bcl-2 expression. Andrographolide may be useful as a potent and selective agent in the treatment of human gastric cancers.
穿心莲内酯已被证明对不同的癌细胞系具有抗癌活性，这些癌细胞系代表不同类型的人类癌症。本研究旨在探讨穿心莲内酯对 BGC-823 人胃癌细胞系的抗癌和促凋亡作用。
采用 MTT 法检测细胞增殖及 IC50，流式细胞术检测细胞周期，Annexin-V/碘化丙啶双染法检测细胞凋亡，透射电镜观察细胞形态学变化，免疫组化和 RT-PCR 检测 Bcl-2、Bax 和 caspase-3 的表达。
穿心莲内酯对 BGC-823 细胞生长具有时间和浓度依赖性抑制作用。与对照组相比，经穿心莲内酯处理 48 小时后，G0-G1 期细胞数量明显增加，S 和 G2-M 期细胞减少，早期和晚期凋亡率均明显高于对照组，且呈浓度依赖性。Bax 和 caspase-3 的表达明显增加，Bcl-2 的表达明显降低。
穿心莲内酯通过上调 Bax 和 caspase-3 的表达，下调 Bcl-2 的表达，抑制 BGC-823 细胞生长并诱导 BGC-823 细胞凋亡。穿心莲内酯可能是治疗人类胃癌的一种有效而选择性的药物。
