Heiman A S, Crews F T
Biochem Biophys Res Commun. 1985 Jul 31;130(2):640-5. doi: 10.1016/0006-291x(85)90465-6.
Purified rat peritoneal mast cells were incubated overnight with or without hydrocortisone (3 X 10(-6) M) and then stimulated with anti-IgE, somatostatin or a phorbol ester-ionophore combination, i.e., 12-O-tetradecanoyl-phorbol-13-acetate and A23187. The release of both histamine and [1-14C]arachidonic acid and its metabolites was determined. Hydrocortisone treatment markedly inhibited both anti-IgE and TPA-A23187 stimulated release, but not release stimulated by somatostatin. These results suggest that anti-inflammatory steroids may alter histamine release through an action involving the activation of the phosphatidylserine-calcium dependent protein kinase or its substrates.
将纯化的大鼠腹膜肥大细胞在有或无氢化可的松（3×10⁻⁶ M）的情况下孵育过夜，然后用抗IgE、生长抑素或佛波酯-离子载体组合（即12-O-十四烷酰佛波醇-13-乙酸酯和A23187）进行刺激。测定组胺和[1-¹⁴C]花生四烯酸及其代谢产物的释放。氢化可的松处理显著抑制抗IgE和TPA-A23187刺激的释放，但不抑制生长抑素刺激的释放。这些结果表明，抗炎类固醇可能通过涉及磷脂酰丝氨酸-钙依赖性蛋白激酶或其底物激活的作用来改变组胺释放。
