Kantner R M, Kirby M L, Goldstein B D
Brain Res. 1985 Jul 8;338(1):196-9. doi: 10.1016/0006-8993(85)90268-9.
Substance P has been implicated as a neuromediator of nociception in the dorsal horn of the spinal cord. This is the first report of quantitative increases in substance P following an acute nociceptive stimulus. Female Wistar rats were injected subcutaneously in the plantar aspect of the right hindpaw with 5% formalin. Substance P levels were determined by quantitative immunohistochemistry 1 h after the injection. The present results show that substance P levels in the upper two laminae of the ipsi-and contralateral dorsal horn were significantly increased 1 h after the formalin injection. These results suggest a direct functional relationship between substance P levels in the upper dorsal horn and an acute chemogenic nociceptive stimulus.
P物质被认为是脊髓背角伤害感受的神经介质。这是首次关于急性伤害性刺激后P物质定量增加的报告。将雌性Wistar大鼠右后爪足底皮下注射5%福尔马林。注射1小时后通过定量免疫组织化学测定P物质水平。目前的结果表明，福尔马林注射1小时后，同侧和对侧背角上两层的P物质水平显著增加。这些结果表明背角上部的P物质水平与急性化学性伤害性刺激之间存在直接的功能关系。
