Jagirdar J, Ishak K G, Colombo M, Brambilla C, Paronetto F
Cancer. 1985 Oct 1;56(7):1643-8. doi: 10.1002/1097-0142(19851001)56:7<1643::aid-cncr2820560730>3.0.co;2-o.
Fibronectin, a high molecular weight glycoprotein component of the extracellular matrix, was localized extracellularly and intracellularly in the nuclei and the cytoplasm of hepatocellular carcinomas (HCC) by the use of the biotin-avidin method on paraffin-embedded, pepsin-treated tissue. The specificity of the staining was confirmed by absorbing the antibodies with purified fibronectin. Twenty HCC were investigated (7 were autopsy specimens and 13 biopsy and surgical specimens). The fibronectin was either increased or decreased. When increased the fibronectin consisted of thin or thick feathery strands and was either pericellular, sinusoidal, periglandular, or stromal. Marked increase of fibronectin was seen in fibrolamellar HCC, clear cell HCC, and encapsulated HCC, correlating with the better prognosis. Decreased fibronectin was seen in two undifferentiated HCC. HCC obtained at autopsy displayed nuclear and cytoplasmic fibronectin staining. Nuclear fibronectin may be an expression of the DNA binding ability of fibronectin which becomes demonstrable in cells with increased membrane permeability.
纤连蛋白是细胞外基质的一种高分子量糖蛋白成分，通过对石蜡包埋、胃蛋白酶处理的组织采用生物素-抗生物素蛋白法，在肝细胞癌（HCC）的细胞核和细胞质内及细胞外均检测到其存在。用纯化的纤连蛋白吸收抗体，证实了染色的特异性。对20例HCC进行了研究（7例为尸检标本，13例为活检和手术标本）。纤连蛋白含量有增加或减少的情况。当纤连蛋白增加时，它由细或粗的羽毛状条索组成，位于细胞周围、窦状隙、腺泡周围或间质中。在纤维板层型HCC、透明细胞HCC和包膜型HCC中可见纤连蛋白显著增加，这与较好的预后相关。在2例未分化HCC中纤连蛋白减少。尸检获得的HCC显示有细胞核和细胞质纤连蛋白染色。细胞核纤连蛋白可能是纤连蛋白DNA结合能力的一种表现，在膜通透性增加的细胞中可显现出来。
