Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Prostate. 2014 Jan;74(1):61-9. doi: 10.1002/pros.22730. Epub 2013 Sep 21.
The goal of the Prostate Cancer Biorepository Network (PCBN) is to develop a biorepository with high-quality, well-annotated specimens obtained in a systematic, reproducible fashion using optimized and standardized protocols, and an infrastructure to facilitate the growth of the resource and its wide usage by the prostate cancer research community. An emerging area of concern in the field of prostate cancer biobanking is an apparent shift in the proportion of surgical procedures performed for prostate cancer treatment from radical retropubic prostatectomy (RRP) to robot-assisted laparoscopic prostatectomy (RALP). Our study aimed to determine the potential impact of the RALP procedure on the detection of known prostate cancer biomarkers, and the subsequent suitability of RALP-derived specimens for prostate cancer biomarker studies.
DNA and RNA were extracted from RRP and RALP specimens. Quality assessment was conducted using spectrophotometric analysis and RNA was analyzed for RNA integrity number (RIN) and by real-time reverse-transcription PCR (qRT-PCR) for racemase, hepsin, ERG, TMPRSS2-ERG gene fusions, and the microRNAs miR-26a, miR-26b, miR-141, and miR-221.
We demonstrate that extraction of derivatives from frozen tissues from RRP and RALP specimens yields samples of equally high quality as assessed by spectrophotometric and RIN analysis. Likewise, expression levels of genes analyzed by qRT-PCR did not differ between RRP and RALP-derived tissues.
Our studies indicate that samples obtained from RALP specimens may be suitable for prostate cancer biomarker studies-an important finding given the current shift in surgical procedures for prostate cancer treatment.
前列腺癌生物库网络（PCBN）的目标是开发一个高质量、注释良好的生物库，这些标本是通过系统、可重复的方式使用优化和标准化的方案获得的，并且有一个基础设施来促进资源的增长及其被前列腺癌研究界广泛使用。在前列腺癌生物库领域，一个令人关注的新兴问题是前列腺癌治疗的手术方式明显从根治性前列腺切除术（RRP）转向机器人辅助腹腔镜前列腺切除术（RALP）。我们的研究旨在确定 RALP 手术对已知前列腺癌生物标志物检测的潜在影响，以及随后 RALP 衍生标本是否适合前列腺癌生物标志物研究。
从 RRP 和 RALP 标本中提取 DNA 和 RNA。使用分光光度分析进行质量评估，并通过 RNA 完整性数（RIN）和实时逆转录聚合酶链反应（qRT-PCR）分析用于检测 racemase、hepsin、ERG、TMPRSS2-ERG 基因融合以及 microRNAs miR-26a、miR-26b、miR-141 和 miR-221。
我们证明，从 RRP 和 RALP 标本的冷冻组织中提取衍生物，其质量与分光光度和 RIN 分析评估的质量一样高。同样，qRT-PCR 分析的基因表达水平在 RRP 和 RALP 衍生组织之间没有差异。
我们的研究表明，从 RALP 标本获得的样本可能适合前列腺癌生物标志物研究——鉴于目前前列腺癌治疗的手术方式发生了变化，这是一个重要的发现。
