Withers T Ryan, Yin Yeshi, Yu Hongwei D
Department of Biochemistry and Microbiology, Joan C. Edwards School of Medicine at Marshall University, Huntington, WV, USA.
Pathog Dis. 2014 Mar;70(2):185-8. doi: 10.1111/2049-632X.12102. Epub 2013 Nov 5.
In this study, we performed whole-genome complementation using a PAO1-derived cosmid library, coupled with in vitro transposon mutagenesis, to identify gene locus PA1494 as a novel inhibitor of alginate overproduction in P. aeruginosa strains possessing a wild-type mucA.
在本研究中,我们使用源自PAO1的黏粒文库进行全基因组互补,并结合体外转座子诱变,以鉴定基因位点PA1494是具有野生型mucA的铜绿假单胞菌菌株中藻酸盐过度产生的新型抑制剂。