CIRAD, UMR CMAEE, F-97490 Sainte-Clotilde, La Réunion, France; INRA, UMR 1309 CMAEE, F-34398 Montpellier, France; CRVOI, F-97490 Sainte Clotilde, La Réunion, France.
J Virol Methods. 2014 Jan;195:92-9. doi: 10.1016/j.jviromet.2013.10.001. Epub 2013 Oct 11.
In recent years, Madagascar and the Comoros archipelago have been affected by epidemics of Rift Valley fever (RVF), however detection of Rift Valley fever virus (RVFV) in zebu, sheep and goats during the post epidemic periods was frequently unsuccessful. Thus, a highly sensitive real-time RT-PCR assay was developed for the detection of RVFV at low viral loads. A new RVF SYBR Green RT-PCR targeting the M segment was tested on serum from different RVF seronegative ruminant species collected from May 2010 to August 2011 in Madagascar and the Comoros archipelago and compared with a RVF specific quantitative real time RT-PCR technique, which is considered as the reference technique. The specificity was tested on a wide range of arboviruses or other viruses giving RVF similar clinical signs. A total of 38 out of 2756 serum samples tested positive with the new RT-PCR, whereas the reference technique only detected 5 out of the 2756. The described RT-PCR is an efficient diagnostic tool for the investigation of enzootic circulation of the RVF virus. It allows the detection of low viral RNA loads adapted for the investigations of reservoirs or specific epidemiological situations such as inter-epizootic periods.
近年来，马达加斯加和科摩罗群岛受到裂谷热（RVF）的流行影响，但在疫情后时期，牛、绵羊和山羊中裂谷热病毒（RVFV）的检测常常不成功。因此，开发了一种高灵敏度的实时 RT-PCR 检测方法，用于检测低病毒载量的 RVFV。一种针对 M 片段的新型 RVF SYBR Green RT-PCR 在 2010 年 5 月至 2011 年 8 月期间从马达加斯加和科摩罗群岛收集的不同 RVF 血清阴性反刍动物物种的血清上进行了测试，并与 RVF 特异性定量实时 RT-PCR 技术进行了比较，该技术被认为是参考技术。在广泛的虫媒病毒或其他引起 RVF 类似临床症状的病毒上进行了特异性测试。新 RT-PCR 检测出 38 份来自 2756 份血清样本呈阳性，而参考技术仅在 2756 份中检测到 5 份。所述 RT-PCR 是一种用于调查 RVF 病毒地方性循环的有效诊断工具。它可以检测到低病毒 RNA 载量，适用于研究储主或特定的流行病学情况，如间流行期。
