Bravo C, García-Baro J A, Sobrino J A
Rev Esp Fisiol. 1985 Jun;41(2):243-8.
The effects of microelectrophoretic application of sulphydryl reagents at both the endplate region and extrasynaptic sites have been studied in the mouse diaphragm neuromuscular preparation. Two groups of sulphydryl reagents have been tested: oxidizing agents and reducing agents. Reagents were applied iontophoretically by means of a microelectrophoretic programmer with constant current source. Both groups of sulphydryl reagents elicit depolarizations only when applied at the endplate region. Presynaptic components were ruled out by addition of magnesium chloride to the bathing medium. After blockade of neuromuscular transmission, the depolarizing effect of both groups of sulphydryl reagents persisted. The present results suggest the presence of reactive disulphide bonds and SH groups in the receptor complex of the mammalian neuromuscular junction. These groups could be located at the alpha subunit of the acetylcholine receptor.
在小鼠膈神经肌肉标本中，研究了在终板区和突触外部位微电泳施加巯基试剂的效果。测试了两组巯基试剂：氧化剂和还原剂。通过带有恒流源的微电泳程序器以离子电泳方式施加试剂。两组巯基试剂仅在施加于终板区时才引起去极化。通过向浴液中添加氯化镁排除了突触前成分。在神经肌肉传递被阻断后，两组巯基试剂的去极化作用仍然存在。目前的结果表明，在哺乳动物神经肌肉接头的受体复合物中存在反应性二硫键和巯基。这些基团可能位于乙酰胆碱受体的α亚基上。
