Dept of Pathology, Section of Hematopathology, University of Chicago Medical Center, TW055/MC0008, 5841 S Maryland Ave, Chicago, IL 60637;
Am J Clin Pathol. 2013 Nov;140(5):686-92. doi: 10.1309/AJCPUY0ZMG3VTLFG.
To examine the utility of CD11c expression on monocytes in normal controls and patients with chronic myelomonocytic leukemia (CMML) (n = 23) with flow cytometric immunophenotyping.
Twenty-three CMML samples and 10 control bone marrows submitted for lymphoma staging without evidence of disease were examined.
Monocytes in CMML samples ranged from 4% to 35%. Expression of at least one aberrant monocytic marker was found on the monocytes in 18 (82%) of 22 evaluable cases. The most common aberrancy was underexpression of CD11c (n = 15), while none of the bone marrow controls showed underexpression of CD11c.
A distinct heterogeneous population of monocytic cells with underexpression of CD11c was identified in all these cases. CD11c underexpression was independent of other aberrancies, including HLA-DR underexpression (n = 14), aberrant CD56 expression (n = 11), and underexpression of CD33, CD38, and CD14 (n = 6, 5, and 5, respectively), supporting the utility of CD11c expression status on monocytes in establishing a CMML diagnosis.
通过流式细胞免疫表型分析，研究 CD11c 在正常对照者和慢性粒单核细胞白血病（CMML）（n=23）患者单核细胞中的表达情况。
检测了 23 例 CMML 样本和 10 例无疾病证据的淋巴瘤分期骨髓标本。
CMML 样本中的单核细胞占比为 4%至 35%。在 22 例可评估病例中，有 18 例（82%）的单核细胞至少表达一种异常的单核细胞标志物。最常见的异常是 CD11c 表达下调（n=15），而骨髓对照无一例显示 CD11c 表达下调。
所有这些病例中均发现 CD11c 表达下调的独特异质性单核细胞群体。CD11c 表达下调与其他异常无关，包括 HLA-DR 表达下调（n=14）、异常 CD56 表达（n=11）、CD33、CD38 和 CD14 表达下调（n=6、5 和 5），支持单核细胞 CD11c 表达状态在建立 CMML 诊断中的应用。
