School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, United Kingdom.
PLoS One. 2013 Oct 4;8(10):e75724. doi: 10.1371/journal.pone.0075724. eCollection 2013.
In Arabidopsis, and other plants, the RABA GTPases (orthologous to the Rab11a of mammals) have expanded in number and diversity and have been shown to belong to eight sub clades, some of which have been implicated in controlling vesicles that traffic cell wall polymers and enzymes that synthesise or modify them to the cell wall. In order to investigate this, we have investigated whether T-DNA insertion knockouts of individual RABA genes belonging to different sub clades, impact on the composition of the plant cell wall. Single gene knockouts of the RABA1, RABA2 and RABA4 sub clades primarily affected the percentage composition of pectin, cellulose and hemicellulose within the cell wall, respectively, despite having no obvious phenotype in the whole plant. We hypothesise that vesicles carrying specific types of cargoes from the Golgi to the cell surface may be regulated by particular sub types of RABA proteins, a finding that could have wider implications for how trafficking systems work and could be a useful tool in cell wall research and other fields of plant biology.
在拟南芥和其他植物中，RABA GTPases（与哺乳动物的 Rab11a 同源）数量和多样性增加，并被证明属于八个亚科，其中一些与控制运输细胞壁聚合物和合成或修饰细胞壁的酶的囊泡有关。为了研究这一点，我们研究了属于不同亚科的单个 RABA 基因的 T-DNA 插入敲除是否会影响植物细胞壁的组成。尽管在整个植物中没有明显的表型，但 RABA1、RABA2 和 RABA4 亚科的单个基因敲除主要影响细胞壁中果胶、纤维素和半纤维素的百分比组成。我们假设，从高尔基体到质膜运输特定类型货物的囊泡可能受到特定类型的 RABA 蛋白的调节，这一发现可能对运输系统的工作方式有更广泛的影响，并可能成为细胞壁研究和植物生物学其他领域的有用工具。
