Manchester Pharmacy School, University of Manchester , Stopford Building, Oxford Road, Manchester M13 9PT, United Kingdom.
J Proteome Res. 2013 Dec 6;12(12):5934-42. doi: 10.1021/pr400279u. Epub 2013 Oct 31.
QconCAT is a tool for quantitative proteomics, consisting of an artificial protein, expressed from an artificial gene, made up of a concatenated string of proteotypic peptides selected from the proteins under study. Isotopically labeled QconCAT (usually containing (13)C6-arginine and (13)C6-lysine) provides a standard for each proteotypic peptide included in its sequence. In practice, some QconCAT proteins fail to express at sufficient levels for the purpose of quantitative analysis. Two complementary methods are presented to express recalcitrant QconCAT proteins intended to quantify human hepatic enzymes and transporters.
QconCAT 是一种定量蛋白质组学工具，由一种人工合成的蛋白质组成，该蛋白质由从研究蛋白中选择的肽段的串联序列组成。同位素标记的 QconCAT（通常含有 (13)C6-精氨酸和 (13)C6-赖氨酸）为其序列中包含的每个肽段提供了一个标准。在实践中，一些 QconCAT 蛋白质的表达水平不足以进行定量分析。本文提出了两种互补的方法来表达用于定量分析人类肝酶和转运体的顽固 QconCAT 蛋白质。
