Immunoblotting and ELISA analysis of HSV-1 and HSV-2-specified polypeptides by using an immunoblocking method.

Herpes Simplex Virus (HSV) specified polypeptide antigens were analyzed by immunoblotting and enzyme-linked immunosorbent assay (ELISA) methods. In general, the virus specified proteins showed marked cross-reactivity. However, the HSV-1 specified glycoprotein C could be detected by HSV-1 infected rabbit antiserum only. Two low molecular weight polypeptides showed considerable reactivity with HSV-2 induced immune sera as revealed by an immunoblocking modification of the immunoblotting method. These proteins may prove useful in the serological diagnosis of HSV Type 1 and Type 2 infections.