Quaroni A
Dev Biol. 1985 Oct;111(2):280-92. doi: 10.1016/0012-1606(85)90483-x.
A panel of monoclonal antibodies to intestinal cell surface components has been used to compare the expression of differentiation-specific antigens in the epithelial cells of fetal, suckling, and adult rat small intestine. Indirect immunofluorescence staining, and immunopurification of detergent-solubilized membrane proteins, followed by single- and two-dimensional slab gel electrophoretic analysis, have demonstrated that fetal intestinal cells (at day 21 of gestation) express most differentiation-specific markers typical of adult absorptive villus cells. A marked heterogeneity in antigen expression was observed among different villus cell populations in suckling rat intestine, and three cell surface components were identified which are exclusively present during this period of intestinal development. Striking changes in the patterns of antigen expression in crypt and villus cells, and variations in the apparent isoelectric points for most luminal membrane components, were associated with the maturation of the intestinal mucosa at weaning. These changes could not be prematurely induced by cortisone injection in newborn rats, suggesting that factors other than glucocorticoids are responsible for the postnatal development of the intestinal epithelium. These results suggest that basic differences in biological properties and regulatory mechanisms exist among intestinal epithelial cells at different stages of pre- and postnatal maturation.
一组针对肠细胞表面成分的单克隆抗体已被用于比较胎鼠、乳鼠和成年大鼠小肠上皮细胞中分化特异性抗原的表达。通过间接免疫荧光染色以及对去污剂溶解的膜蛋白进行免疫纯化，随后进行一维和二维平板凝胶电泳分析，结果表明胎鼠小肠细胞（妊娠第21天）表达了大多数成年吸收性绒毛细胞典型的分化特异性标志物。在乳鼠小肠不同绒毛细胞群体中观察到抗原表达存在显著异质性，并且鉴定出三种在此肠道发育阶段特有的细胞表面成分。隐窝细胞和绒毛细胞中抗原表达模式的显著变化，以及大多数腔面膜成分表观等电点的变化，与断奶时肠黏膜的成熟相关。这些变化不能通过给新生大鼠注射可的松过早诱导，这表明除糖皮质激素外的其他因素负责肠上皮的产后发育。这些结果表明，在出生前和出生后成熟的不同阶段，肠上皮细胞在生物学特性和调节机制上存在基本差异。
