Malo C, Ménard D
Biol Neonate. 1983;44(3):177-84. doi: 10.1159/000241712.
The interactions between insulin, cortisone and thyroxine on the functional maturation of the small intestine were investigated. 8-day-old suckling mice received one injection/day during 3 days of a single hormone or of the following hormonal combinations: insulin plus cortisone or insulin plus thyroxine. The differentiation of intestinal epithelial cells was evaluated by measuring brush border membrane hydrolytic activities and proliferation by establishing the mitotic index and 3H-thymidine incorporation into DNA. The effects of cortisone and insulin on enterocyte's differentiation are additive. Thyroxine accelerates the differentiation and has a limited action on cell proliferation. Insulin exerts a sustained effect on these two aspects of the intestinal maturation and, furthermore, acts in synergism with thyroxine. It appears that insulin, cortisone and thyroxine have their own specific effect on differentiation and proliferation of the epithelial cells and that these three hormones act in cooperation to induce the maturation of the suckling mouse small intestine during postnatal development.
研究了胰岛素、可的松和甲状腺素对小肠功能成熟的相互作用。8日龄的乳鼠在3天内每天接受一次单一激素或以下激素组合的注射：胰岛素加可的松或胰岛素加甲状腺素。通过测量刷状缘膜水解活性来评估肠上皮细胞的分化，并通过建立有丝分裂指数和将3H-胸腺嘧啶核苷掺入DNA来评估细胞增殖。可的松和胰岛素对肠上皮细胞分化的作用是相加的。甲状腺素加速分化，对细胞增殖的作用有限。胰岛素对肠道成熟的这两个方面都有持续作用，此外，还与甲状腺素协同作用。胰岛素、可的松和甲状腺素似乎对上皮细胞的分化和增殖有各自特定的作用，并且这三种激素在产后发育过程中协同作用以诱导乳鼠小肠成熟。
