Department of Ophthalmology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Jpn J Ophthalmol. 2014 Jan;58(1):100-9. doi: 10.1007/s10384-013-0282-4. Epub 2013 Oct 16.
We investigated the effects of unoprostone on neurite extension of cultured retinal pieces and axonal regeneration of retinal ganglion cells in the crushed optic nerve of adult cats.
The retinal pieces were cultured with unoprostone or its primary metabolite, M1, dissolved in DMSO or polysorbate for 14 days, and the number and length of Tau-1-positive neurites and glial processes labeled with anti-glial fibrillary acidic protein antibodies were examined. After the optic nerve was crushed, unoprostone was injected into the vitreous body and the crushed site. On day 12, wheat germ agglutinin-conjugated horseradish peroxidase was injected into the vitreous body to anterogradely label the regenerated axons. On day 14, the optic nerve was excised and longitudinally sectioned. After peroxidase reaction, the number of axons regenerating beyond the crush site was examined.
The greatest number of neurites protruded from the cultured retinal pieces in 3 μM unoprostone and 3 μM M1. The neurite length was also the longest at 3 μM unoprostone and 3 μM M1, in which no glial processes were detected. After injections of 3 μM unoprostone, the final concentration in the vitreous humor, into the vitreous body and the crush site, the optic nerve fibers regenerated and extended beyond the crush site. In contrast, almost no fibers extended beyond the crush site after injection of phosphate-buffered saline.
The results indicate that intravitreal injection of unoprostone promotes regeneration of crushed optic nerve fibers in adult cats.
我们研究了前列腺素 F2α类似物 unoprostone 对培养的视网膜片的神经突延伸和成年猫视神经挤压后视网膜神经节细胞轴突再生的影响。
视网膜片用溶解在 DMSO 或聚山梨醇酯中的 unoprostone 或其主要代谢物 M1 培养 14 天，用抗神经微丝蛋白抗体标记 Tau-1 阳性神经突和神经胶质过程的数量和长度进行检查。视神经挤压后，将 unoprostone 注入玻璃体和挤压部位。第 12 天，将麦胚凝集素结合辣根过氧化物酶注入玻璃体以顺行标记再生的轴突。第 14 天，取出视神经并进行纵向切片。过氧化物酶反应后，检查超过挤压部位再生的轴突数量。
在 3μM unoprostone 和 3μM M1 中，培养的视网膜片中突出的神经突数量最多。在 3μM unoprostone 和 3μM M1 中，神经突长度也最长，且未检测到神经胶质过程。注射 3μM unoprostone 后，玻璃体中的最终浓度，注入玻璃体和挤压部位，视神经纤维再生并延伸超过挤压部位。相比之下，注射磷酸盐缓冲盐水后，几乎没有纤维延伸超过挤压部位。
结果表明，玻璃体内注射前列腺素 F2α类似物 unoprostone 可促进成年猫视神经挤压后纤维的再生。
