Huwyler T, Hirt A, Felix D, Morell A
Int J Immunopharmacol. 1985;7(4):573-6. doi: 10.1016/0192-0561(85)90079-7.
We studied the influence of various extracellular Ca++ and Mg++ concentrations on human natural killer (NK) cells and found that NK cell-mediated target cell lysis requires the presence of both divalent cations. The calcium channel blocker verapamil and the local anaesthetics procaine and lidocaine inhibited NK cell-mediated killing of K 562 target cells, whereas the selective K+ channel blocker tetraethylammonium and the selective Na+ channel blocker tetrodotoxin had no effect on NK activity. Our results demonstrate an important role of extracellular Ca++ and Mg++ for NK cell-mediated killing and suggest that a free transmembrane Ca++ passage is required for target cell lysis.
我们研究了不同细胞外钙离子（Ca++）和镁离子（Mg++）浓度对人自然杀伤（NK）细胞的影响，发现NK细胞介导的靶细胞裂解需要两种二价阳离子的存在。钙通道阻滞剂维拉帕米、局部麻醉药普鲁卡因和利多卡因抑制了NK细胞介导的对K562靶细胞的杀伤作用，而选择性钾离子（K+）通道阻滞剂四乙铵和选择性钠离子（Na+）通道阻滞剂河豚毒素对NK活性没有影响。我们的结果证明了细胞外Ca++和Mg++在NK细胞介导的杀伤中起重要作用，并表明靶细胞裂解需要自由的跨膜钙离子通道。
