Hanley D A, Wellings P G
J Immunoassay. 1985;6(3):245-59. doi: 10.1080/01971528508063032.
A radioimmunoassay for Parathyroid Hormone which is used in a clinical setting was characterized by immunoreactivity with various synthetic fragments of the hormone, serum parathyroid hormone response to oral calcium intake in normocalcemic calcium stone-formers, and ability to detect fragments of parathyroid hormone secreted by abnormal human parathyroid tissue in vitro. Although almost all of the recognition sites for the antiserum were within the 53-84 carboxyl terminal amino acid sequence of the hormone, the radioimmunoassay mainly detected the "intact" hormone rather than the carboxyl terminal fragment(s) which most "carboxyl-terminal" assays of parathyroid hormone are claimed to preferentially detect. Differences in tertiary structure between the intact hormone and its fragments probably account for the relative inability of this antiserum to detect the carboxyl terminal fragment(s).
一种用于临床的甲状旁腺激素放射免疫测定法的特点是，它能与该激素的各种合成片段发生免疫反应，能检测正常血钙水平的含钙结石形成者口服钙后血清甲状旁腺激素的反应，还能在体外检测异常人类甲状旁腺组织分泌的甲状旁腺激素片段。尽管抗血清的几乎所有识别位点都在该激素53 - 84位羧基末端氨基酸序列内，但该放射免疫测定法主要检测的是“完整”激素，而非大多数声称优先检测羧基末端片段的甲状旁腺激素“羧基末端”测定法所检测的羧基末端片段。完整激素与其片段之间三级结构的差异可能是导致这种抗血清相对无法检测羧基末端片段的原因。
