Gohill J, Cary P D, Couppez M, Fritzler M J
J Immunol. 1985 Nov;135(5):3116-21.
The sera of patients with systemic lupus erythematosus (SLE) and drug-induced lupus (DIL) were used to study the antigenic epitopes on nuclear histones that bind antibodies in these sera. ELISA and immunoblotting techniques showed that antibodies from both patient groups bound all classes of intact histone: H1 greater than H2B greater than H2A greater than H3 greater than H4. The different classes of histone were enzymatically or chemically cleaved to produce a series of peptide fragments which were then used to map the reactive epitopes by ELISA and immunoblotting. Ten of 11 DIL sera and 11 of 12 SLE sera bound the carboxy and amino terminal peptides. Only one sera of each group bound to the central hydrophobic polypeptide. The reactivity of DIL sera with fractionated histone polypeptides was similar to that observed with SLE sera. This observation suggests that the histone epitopes reacting with DIL sera are no less restricted than those reacting with SLE.
系统性红斑狼疮（SLE）和药物性狼疮（DIL）患者的血清被用于研究与这些血清中抗体结合的核组蛋白上的抗原表位。酶联免疫吸附测定（ELISA）和免疫印迹技术表明，两个患者组的抗体均能与所有完整组蛋白类别结合：H1＞H2B＞H2A＞H3＞H4。对不同组别的组蛋白进行酶切或化学切割，以产生一系列肽片段，然后通过ELISA和免疫印迹来绘制反应性表位图谱。11份DIL血清中的10份以及12份SLE血清中的11份与羧基端和氨基端肽段结合。每组仅一份血清与中央疏水多肽结合。DIL血清与分级分离的组蛋白多肽的反应性与SLE血清观察到的反应性相似。这一观察结果表明，与DIL血清发生反应的组蛋白表位的受限程度并不低于与SLE血清发生反应的组蛋白表位。
