Brinet A, Fournel C, Faure J R, Venet C, Monier J C
Laboratoire d'Immunologie, UFR Alexis Carrel, Lyon, France.
Clin Exp Immunol. 1988 Oct;74(1):105-9.
In the canine systemic lupus erythematosus (SLE), anti-double stranded DNA (ds-DNA) antibodies (enzyme linked immunosorbent assay (ELISA) or indirect immunofluorescence on Crithidia luciliae) are rare whereas anti-histone antibodies are often found: 61.7% with ELISA and 74% with immunoblot. In canine SLE the pattern of anti-histone antibodies on immunoblot is different from anti-histone antibodies in human SLE. Indeed, histone fractions which are most often recognized by the canine antibodies are by order of frequency H3, H4 and H2A, whereas in man this order is H1, H2B then H3. In the diagnostic criteria of canine SLE, we suggest replacing the anti-ds-DNA antibodies by the anti-histone antibodies detected by immunoblot.
在犬类系统性红斑狼疮（SLE）中，抗双链DNA（ds-DNA）抗体（通过酶联免疫吸附测定（ELISA）或在利什曼原虫上进行间接免疫荧光检测）很少见，而抗组蛋白抗体则经常被检测到：ELISA检测阳性率为61.7%，免疫印迹检测阳性率为74%。在犬类SLE中，免疫印迹上抗组蛋白抗体的模式与人类SLE中的抗组蛋白抗体不同。实际上，犬类抗体最常识别的组蛋白组分按频率依次为H3、H4和H2A，而在人类中，这个顺序是H1、H2B然后是H3。在犬类SLE的诊断标准中，我们建议用免疫印迹检测到的抗组蛋白抗体取代抗ds-DNA抗体。
