嵌合腺病毒的制备。
Production of chimeric adenovirus.
作者信息
Miralles Marta, Garcia Marc, Tejero Marcos, Bosch Assumpció, Chillón Miguel
机构信息
Department of Biochemistry and Molecular Biology, Center of Animal Biotechnology and Gene Therapy (CBATEG), Universitat Autonoma Barcelona, Bellaterra, Spain.
出版信息
Methods Mol Biol. 2014;1089:231-43. doi: 10.1007/978-1-62703-679-5_16.
The use of chimeric pseudotyped vectors is a common way to modify the adenoviral tropism by replacing the fiber protein. In this chapter the procedure to generate a chimeric adenovirus pre-stock from a plasmid containing the adenoviral genome is described. Also, the chimeric adenovirus replicative cycle to increase the yield in further productions is determined. Finally, two different protocols, in culture plates and in suspension cultures, to produce the virus at large scale are also detailed.
使用嵌合假型载体是通过替换纤维蛋白来改变腺病毒嗜性的常用方法。在本章中，描述了从含有腺病毒基因组的质粒产生嵌合腺病毒预储备的程序。此外，还确定了用于在进一步生产中提高产量的嵌合腺病毒复制周期。最后，还详细介绍了在培养板和悬浮培养中大规模生产病毒的两种不同方案。
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