Institute of Chemical Science and Engineering, Institute of Bioengineering, National Centre of Competence in Research NCCR Chemical Biology, École Polytechnique Fédérale de Lausanne, EPFL SB ISIC LIP, 1015 Lausanne (Switzerland); Current address: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139 (USA).
Chembiochem. 2013 Nov 25;14(17):2239-42. doi: 10.1002/cbic.201300472. Epub 2013 Oct 16.
Mycobacterium goes yeast: Target deconvolution of anti-tuberculosis drugs can be a very challenging task. Here we report a yeast 3-hybrid system that allows promising small molecules to be screened for protein targets of a pathogen in nontoxic yeast cells. The system employs libraries of randomly fragmented bacterial DNA and offers a technically simple alternative approach for target identification.
抗结核药物的靶标解析可能是一项极具挑战性的任务。在这里，我们报告了一个酵母三杂交系统，该系统允许在无毒的酵母细胞中筛选有前途的小分子，以鉴定病原体的蛋白质靶标。该系统使用随机片段化的细菌 DNA 文库，为靶标鉴定提供了一种技术简单的替代方法。
