在Cobas E601和ADVIA Centaur上进行的BNP和NT-proBNP检测的分析性能及心力衰竭研究

Analytical performances and heart failure research of the BNP and NT-proBNP assays on the Cobas E601 and ADVIA Centaur.

作者信息

Zhang Xiuming, Wang Weijia, Wang Qian, Yang Youye, Wen Dongmei, Yang Zhizhao, Lan Haili, Li Fei, Ouyang Nengliang, Kan Lijuan, Suo Minghuan, Yan Haizhong

机构信息

Department of Laboratory Diagnosis, Sun Yat-Sen University, Zhongshan 528403, China.

出版信息

Clin Lab. 2013;59(7-8):715-25.

PMID:24133898

Abstract

BACKGROUND

The analytical performances of the NT-proBNP and BNP assays on the Cobas E601 and ADVIA Centaur were thoroughly evaluated. In addition, the values of BNP and NT-proBNP, which are heart failure markers, were compared in the diagnosis of HF patients with or without acute cerebral infarction since they could also be elevated in ischemic stroke.

METHODS

Clinical and Laboratory Standards Institute (CLSI) documents were employed in the analytical evaluation of NT-proBNP and BNP assays on the Cobas E601 and ADVIA Centaur. Then 100 heart failure patients and 103 cerebral infarction complicated with heart failure patients, who had been diagnosed by clinical doctors blinded to NT-proBNP and BNP concentrations, were chosen to compare their values in the diagnosis of heart failure with or without acute cerebral infarction.

RESULTS

The NT-proBNP and BNP methods are precise and accurate (total CV < 2.9%, deviation < 3.6%), have wide dynamic measuring ranges (8 pg/mL to 35 126 pg/mL and 2.0 pg/mL to 5094 pg/mL, respectively) with maximum dilutability of 1:2, and are free of common interferences. The most suitable sample types for NT-proBNP and BNP are serum and EDTA plasma, respectively, and both methods correlate well in simple-HF patients. Unlike BNP, the level of NT-proBNP is much higher in HF patients with acute cerebral infarction (p < 0.001). The Cobas E601 and ADVIA Centaur systems have good analytical performances.

CONCLUSIONS

In HF patients with acute cerebral infarction, the NT-proBNP and BNP levels did not correlate and thus had implications for clinical diagnosis.

摘要

背景

对Cobas E601和ADVIA Centaur上NT-proBNP和BNP检测的分析性能进行了全面评估。此外，由于BNP和NT-proBNP作为心力衰竭标志物，在合并或未合并急性脑梗死的心力衰竭患者诊断中进行了比较，因为它们在缺血性卒中中也可能升高。

方法

采用临床和实验室标准协会（CLSI）文件对Cobas E601和ADVIA Centaur上的NT-proBNP和BNP检测进行分析评估。然后选择100例心力衰竭患者和103例合并心力衰竭的脑梗死患者，这些患者由对NT-proBNP和BNP浓度不知情的临床医生诊断，比较他们在合并或未合并急性脑梗死的心力衰竭诊断中的数值。

结果

NT-proBNP和BNP方法精确且准确（总变异系数<2.9%，偏差<3.6%），具有宽动态测量范围（分别为8 pg/mL至35126 pg/mL和2.0 pg/mL至5094 pg/mL），最大稀释度为1:2，且无常见干扰。NT-proBNP和BNP最适合的样本类型分别为血清和EDTA血浆，两种方法在单纯性心力衰竭患者中相关性良好。与BNP不同，急性脑梗死心力衰竭患者的NT-proBNP水平要高得多（p<0.001）。Cobas E601和ADVIA Centaur系统具有良好的分析性能。