Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, SK-845 38 Bratislava, Slovakia.
Anal Biochem. 2014 Jan 15;445:49-53. doi: 10.1016/j.ab.2013.10.006. Epub 2013 Oct 14.
We have prepared 4-nitrophenyl caffeate by a combination of standard procedures of organic synthesis and enzymatic deacetylation. Based on hydrolysis of 4-nitrophenyl caffeate, a convenient spectrophotometric assay was developed for specific monitoring of caffeoyl esterase. The method is fast and easy to perform, and it requires no expensive equipment. Its reliability was tested on eight enzyme preparations comprising various combinations of caffeoyl, feruloyl, and acetyl esterase as well as protease activities.
我们通过组合标准的有机合成程序和酶脱乙酰化作用制备了 4-硝基苯咖啡酸酯。基于 4-硝基苯咖啡酸酯的水解，我们开发了一种方便的分光光度测定法，用于特异性监测咖啡酰酯酶。该方法快速且易于操作，不需要昂贵的设备。我们在包含各种咖啡酰、阿魏酰和乙酰酯酶以及蛋白酶活性的 8 种酶制剂上测试了该方法的可靠性。
