Estavillo Gonzalo M, Verhertbruggen Yves, Scheller Henrik V, Pogson Barry J, Heazlewood Joshua L, Ito Jun
ARC Centre of Excellence in Plant Energy Biology and Research School of Biology, The Australian National University, Canberra, Australia.
Methods Mol Biol. 2014;1072:453-67. doi: 10.1007/978-1-62703-631-3_31.
The cytosol is the fluid portion of the cell that is not partitioned by membranes. It contains a highly diverse collection of substances and is central to many essential cellular processes ranging from signal transduction, metabolite production and transport, protein biosynthesis and degradation to stress response and defense. Despite its importance, only a few proteomic studies have been performed on the plant cytosol. This is largely due to difficulties in isolating relatively pure samples from plant material free of disrupted organelle material. In this chapter we outline methods for isolating the cytosolic fraction from Arabidopsis cell cultures and seedlings and provide guidance on assessing purity for analysis by mass spectrometry.
胞质溶胶是细胞中未被膜分隔的流体部分。它包含种类极为多样的物质，并且对于许多重要的细胞过程至关重要，这些过程涵盖信号转导、代谢物产生与运输、蛋白质生物合成与降解以及应激反应和防御等。尽管其很重要，但针对植物胞质溶胶仅开展了少数蛋白质组学研究。这主要是因为从植物材料中分离出不含破碎细胞器物质的相对纯样品存在困难。在本章中，我们概述了从拟南芥细胞培养物和幼苗中分离胞质部分的方法，并为通过质谱分析评估纯度提供指导。
