Kaneko Kentaro, Shiraya Takeshi, Mitsui Toshiaki, Nishimura Shin-Ichiro
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, Niigata, Japan.
Methods Mol Biol. 2014;1072:645-53. doi: 10.1007/978-1-62703-631-3_44.
Glycoprotein is a major element in higher organisms including mammalians and plants. It is widely accepted that variation in cellular N-glycome is related to modulation in dynamic cellular mechanisms such as cell-cell adhesion, cell activation, and malignant alterations in mammalian cells. However, the physiological importance of glycan modification of glycoproteins in plant cells is still a matter of dispute. Therefore, a comprehensive and high-throughput analysis of N-glycome in plant glycoproteins is needed. Here, an application of the glycoblotting-mass spectrometry technique to plant glycoprotein research is described.
糖蛋白是包括哺乳动物和植物在内的高等生物中的主要成分。人们普遍认为，细胞N-聚糖组的变化与动态细胞机制的调节有关，如哺乳动物细胞中的细胞间粘附、细胞激活和恶性改变。然而，植物细胞中糖蛋白糖基修饰的生理重要性仍存在争议。因此，需要对植物糖蛋白中的N-聚糖组进行全面且高通量的分析。本文描述了糖印迹-质谱技术在植物糖蛋白研究中的应用。
